TatianaMolchanovaScientificCareer

Education:
Ph.D. in Biological Sciences, "Hematological Scientific Center" and "Engelhardt Institute of Molecular Biology", Russian Academy of Science, Moscow.
BS, Chemist (Chemistry of the Biologically Active Natural Compounds), Institute of Fine Chemical Technology, Moscow.

Major steps of scientific career:

My scientific career is mostly oriented on different aspects of protein studies. I could be proud that my first achievements had been made in the protein conformational dynamic (1975-1982) when I was working at the Engelhardt Institute of Molecular Biology", Russian Academy of Science, Moscow (http://www.eimb.ru/index.html ). The Leader of the group, which I was working with, was one of the pioneer scientist (Abaturov LV) formulated molten globular protein conformational state. We had multiple opportunities to get “molten globular state” for the Hb and it constituent parts, recorded its properties with different spectroscopy and with studies of the kinetics of its proteolysis. Since that time protein and nucleic acid conformational dynamics became an obvious ruling process in the biological systems. My fundamental knowledge had found the application at the molecular diagnosis of human hereditary diseases mostly hemoglobinopathies. I have made impact making diagnosis for hundreds patients who were suffering from the hereditary anemia, sickle cell disease, thalassemia. I had a chance to work in close contact with Academician Andrey I. Vorobiev (http://www.pmc.ru:8100/data/eng/publ/Stell/vorobjev.html)
Academician Andrey D. Mirzabekov (http://www.eimb.ru/index.html), Professor Alex G. Rumyanzhev in Russia (www.yandex.ru). My best achievements in the diagnosis of hemoglobinopathies were succeeded when I was working with Professor Titus H.J. Huisman, who was a Chairman of the International Center for the Hemoglobinopathies Diagnosis, Augusta, GA, USA. I was in charge for protein structure-base and DNA-based diagnosis of the mutant hemoglobin in patients with different types of clinical manifestations. Samples of blood were sent to our Center from all over the world. Using multiple protein and molecular biological techniques we reviewed the expression of the two alpha globin genes and succeeded with the new conclusions. I was initiated to present on Internet the Professor THJ Huisman’s tremendous collection of the hemoglobin data (http://globin.cse.psu.edu/html/huisman/variants/. Based on our experience we also wrote the Protocols for the mutant hemoglobin diagnosis, which were presented on Internet (http://geocites.com/Augusta/2857/) we are going to restore these protocols on Internet). Later on I was working for the private company, MD, USA. I was not very exited with the way of studies in the Department where I was working for. However I got experience in search of the receptors, receptor-ligand binding, cell cultures, drug screening. My experience found a great application in studies of the Ca2+-binding proteins when I was working with Prof. Jim B. Ames (http://www.carb.nist.gov/ames.html) in the Center for Advanced research in Biotechnology, MD, USA (Ames,J.B., Hamasaki,N., and Molchanova,T. (2002). Structure and calcium-binding studies of a recoverin mutant (E85Q) in an allosteric intermediate state. Biochemistry 41, 5776-5787). (http://www.mc.vanderbilt.edu/biolib/services/rics/alerts/folding0601.rtf ; http://metallo.scripps.edu/analysis/newpdb/recent_pdb.20020802.html)
Currently I am holding a position of the Biologist VII at the NIH/NICHD, Bethesda, MD, USA.

As my major scientific achievements I could consider:

1. The formulation of the conception of the local conformational mobility as a major factor in the protein conformational dynamics and protein stability
Abaturov L.V., Molchanova T.P.: Dynamic structure of proteins according to the data of the hydrogen exchange and proteolytic degradation- In the Book:"Equilibrium Dynamics of the Native Structure of Protein", E.A. Burshtein, ed., Pushino, 1977, pp.5-25 ( in Russian).; Molchanova,T.P., Malozemova,N.G., and Abaturov,L.V. (1982).Proteolytic degradation of native hemoglobin and its constituent parts- -isolated subunits and globin. I. Kinetic data and the character of the process of the breakdown of native forms]. Mol. Biol. (Mosk) 16, 1128-1143.; Abaturov,L.A. and Molchanova,T.P. (1982). Proteolytic degradation of native hemoglobin and its constituent parts- -isolated subunits and globin. II. Correlation with the hydrogen exchange data and the mechanism of the burst-like proteolytic breakdown of native proteins]. Mol. Biol. (Mosk) 16, 1144-1164.; Abaturov L.V., Lebedev Yu.O., Molchanova T.P. et al.: Conformational rigidity and fluctuational motility of the global proteins: water as a stabilizer and plastificator of structure.- In the Book "Equilibrium Dynamics of the Biopolymer Structure", Pushino, Russia, 1990, pp.49-77 (in Russian).

2. Role of the destabilizing mutations in the alpha- and beta-globin chains in the hemoglobin evolution.
Abaturov L.V., Molchanova T.P.: The fewer number of detected alpha chain point mutations in comparison with beta chain mutants of human hemoglobins as a result of the difference in conformational stability of normal chains- XIV International Congress of Genetics, Moscow, 1978, part 1, p. 25.; Tatiana Molchanova [Center for Advanced Research and Biotechnology, University of Maryland, USA]The amino acids that are important for protein stability, are better conserved in evolution: a simple lesson from the study of human hemoglobin mutational database http://www.ncbi.nlm.nih.gov/CBBresearch/Seminar/2001spring/

3. Red Blood Cell membrane proteins 4.1a and 4.1b as a determinant for the Red Blood Cell maturation and Red Blood Cell pathological state
Molchanova, T.P., Kolodey, S.V., Tokarev, Yu.N.: Human erythrocyte membrane proteins 4.1a and 4.1b in terminal stages of erythropoiesis. Abst. Symp. Molecular Factors of Hematopoiesis and Stem Cells, Moscow, 1990, p. 76; Molchanova,T.P., Kolodei,S.V., and Tokarev,Y. (1991). Expression of human membrane proteins 4.1a and 4.1b in reticulocytes and mature erythrocytes. Biomed. Sci. 2, 379-384.

4. Diagnosis of the hereditary hemoglobinopathies through protein and DNA sequencing (more than 60 publications)

5. Diagnosis of a new specific thalassemia mutation in the Russian Nobel Lermontov Family (who are closest alive relatives of famous Russian Poet Michail Yurievich Lermontov, a Prophet; and are also my relatives]
Molchanova,T.P., Postnikov,Y., V, Gu,L.H., and Huisman,T.H. (1998). Historical note: the beta-thalassemia allele in the noble Russian family Lermontov is identified as the ATG-->ACG change in the initiation codon. Hemoglobin 22, 283-286.

6. The mystery of the alpha2- and alpha1-globin gene expression.
Molchanova,T.P., Pobedimskaya,D.D., and Huisman,T.H. (1994). The differences in quantities of alpha2- and alpha1-globin gene variants in heterozygotes. Br. J. Haematol. 88, 300-306.; Molchanova,T.P., Smetanina,N.S., and Huisman,T.H. (1995). A second, elongated, alpha 2-globin mRNA is present in reticulocytes from normal persons and subjects with terminating codon or poly A mutations. Biochem. Biophys. Res. Commun. 214, 1184-1190; Molchanova,T.P. and Huisman,T.H. (1996). The importance of the 3' untranslated region for the expression of the alpha-globin genes. Hemoglobin 20, 41-54.

7. World Wide Web Locus Specific (hemoglobin) databases.
Molchanova T.P., and T.H.J. Huisman: Human hemoglobin variants: a gene spesific database-HUGO Mutation Database MeetingSan Francisco, October, 1996;   Chui,D.H., Hardison,R., Riemer,C., Miller,W., Carver,M.F., Molchanova,T.P., Efremov,G.D., and Huisman,T.H. (1998). An electronic database of human hemoglobin variants on the World Wide Web. Blood 91, 2643-2644. Hardison,R.C., Chui,D.H., Riemer,C.R., Miller,W., Carver,M.F., Molchanova,T.P., Efremov,G.D., and Huisman,T.H. (1998). Access to a syllabus of human hemoglobin variants (1996) via the World Wide Web. Hemoglobin 22, 113-127.

8. Collaborative studies of the intermediate state of the mutant (E85Q) recoverin. Putative Ca2+ binding protein in retina.
Ames,J.B., Hamasaki,N., and Molchanova,T. (2002). Structure and calcium-binding studies of a recoverin mutant (E85Q) in an allosteric intermediate state. Biochemistry 41, 5776-5787.

The most cited Tatiana Molchanova’s publications:

Cited Index: 34
THE DIFFERENCES IN QUANTITIES OF ALPHA-2-GLOBIN AND ALPHA-1-GLOBIN GENE VARIANTS IN HETEROZYGOTES
MOLCHANOVA TP, POBEDIMSKAYA DD, HUISMAN THJ
BRITISH JOURNAL OF HAEMATOLOGY, 88 (2): 300-306 OCT 1994

Cited Index: 26
HB ADANA OR ALPHA(2)59(E8)GLY-]ASP-BETA(2), A SEVERELY UNSTABLE ALPHA(1)-GLOBIN VARIANT, OBSERVED IN COMBINATION WITH THE -(ALPHA)20.5 KB ALPHA-THAL-1 DELETION IN 2 TURKISH PATIENTS
CURUK MA, DIMOVSKI AJ, BAYSAL E, GU LH, KUTLAR F, MOLCHANOVA TP, WEBBER BB, ALTAY C, GURGEY A, HUISMAN THJ
AMERICAN JOURNAL OF HEMATOLOGY, 44 (4): 270-275 DEC 1993

Cited Index: 18
A NEW SCREENING-TEST FOR UNSTABLE HEMOGLOBINS USING N-BUTANOL AND RED-BLOOD-CELLS
MOLCHANOVA TP
HEMOGLOBIN, 17 (1): 81-84 1993

Cited Index: 16
HB GRAZ OR ALPHA-2-BETA-22(NA2)HIS-]LEU - A NEW BETA-CHAIN VARIANT OBSERVED IN 4 FAMILIES FROM SOUTHERN AUSTRIA
LIU JS, MOLCHANOVA TP, GU LH, WILSON JB, HOPMEIER P, SCHNEDL W, BALAUN E, KREJS GJ, HUISMAN THJ
HEMOGLOBIN, 16 (6): 493-501 1992

Cited Index: 16
A NEW ABNORMAL HEMOGLOBIN - HB MOZHAISK BETA-92(F8)HIS-]ARG
SPIVAK VA, MOLCHANOVA TP, POSTNIKOV YV, ASEEVA EA, LUTSENKO IN, TOKAREV YN
HEMOGLOBIN, 6 (2): 169-181 1982

Cited Index: 15
A NEW HEMOGLOBIN-VARIANT - HB DAGESTAN ALPHA-60(E9) LYS-]GLU
SPIVAK VA, MOLCHANOVA TP, ERMAKOV NV, TOKAREY YN, MARTINEZ G, SZELENYI J, HORANYI M, FOLDI J, HOLLAN S, KAZIEVA H, SHAMOV IA
HEMOGLOBIN, 5 (2): 133-138 1981

Cited Index: 13
A SIMPLIFIED PROCEDURE FOR SEQUENCING AMPLIFIED DNA CONTAINING THE ALPHA-2-GLOBIN OR ALPHA-1-GLOBIN GENE
MOLCHANOVA TP, POBEDIMSKAYA DD, POSTNIKOV YV
HEMOGLOBIN. 18 (3): 251-255 1994

Cited Index: 11
HB-ALESHA OR ALPHA(2)BETA(2)67(E11)VAL-]MET - A NEW UNSTABLE HEMOGLOBIN-VARIANT IDENTIFIED THROUGH SEQUENCING OF AMPLIFIED DNA
MOLCHANOVA TP, POSTNIKOV YV, POBEDIMSKAYA DD, SMETANINA NS, MOSCHAN AA, KAZANETZ EG, TOKAREV YN, HUISMAN THJ
HEMOGLOBIN,. 17 (3): 217-225 1993

Cited Index: 11
ALLELE-SPECIFIC AMPLIFICATION FOR THE IDENTIFICATION OF SEVERAL HEMOGLOBIN-VARIANTS
POSTNIKOV YV, MOLCHANOVA TP, HUISMAN THJ
HEMOGLOBIN 17 (5): 439-452 1993

Cited Index: 10
ACCESS TO A SYLLABUS OF HUMAN HEMOGLOBIN VARIANTS (1996) VIA THE WORLD WIDE WEB
HARDISON RC, CHUI DHK, RIEMER CR, MILLER W, CARVER MFH, MOLCHANOVA TP, EFREMOV GD, HUISMAN THJ
HEMOGLOBIN 22 (2): 113-127 1998

Cited Index: 9
COMPOUND HETEROZYGOSITY FOR 2 ALPHA-GLOBIN GENE DEFECTS, HB-TAYBE (ALPHA-1, 38 OR 39 MINUS THR) AND A POLY A MUTATION (ALPHA-2, AATAA(A)UNDER-BAR-]AATAA(G)UNDER-BAR), RESULTS IN A SEVERE HEMOLYTIC-ANEMIA
POBEDIMSKAYA DD, MOLCHANOVA TP, STREICHMAN S, HUISMAN THJ
AMERICAN JOURNAL OF HEMATOLOGY 47 (3): 198-202 NOV 1994

Cited Index: 8
HB BAB-SAADOUN OR ALPHA(2)BETA(2)48(CD7)LEU-]PRO, A MILDLY UNSTABLE VARIANT FOUND IN AN ARABIAN BOY FROM TUNISIA
MOLCHANOVA TP, WILSON JB, GU LH, GUEMIRA F, FATTOUM S, HUISMAN THJ
HEMOGLOBIN, 16 (4): 267-273 1992

Cited Index: 6
HB-TIGRAYE OR ALPHA(2)BETA(2)79(EF3)ASP-]HIS(GAC-]CAC) - A HEMOGLOBIN-VARIANT WITH INCREASED OXYGEN-AFFINITY OBSERVED IN AN ETHIOPIAN MALE
MOLCHANOVA TP, POSTNIKOV YV, GU LH, PRIOR JF, RAVEN JL, BENNETT JA, HUISMAN THJ
HEMOGLOBIN, 17 (3): 247-250 1993

Cited Index: 5
2 DIFFERENT MUTATIONS IN CODON-68 ARE OBSERVED IN HB G-PHILADELPHIA HETEROZYGOTES
MOLCHANOVA TP, POBEDIMSKAYA DD, YE Z, HUISMAN THJ
AMERICAN JOURNAL OF HEMATOLOGY, 45 (4): 345-346 APR 1994

World Wide References for Tatiana Molchanova’s Publications

http://www.haem.net/clinical/clinical014.asp
http://www.dur.ac.uk/~dbl0www3/MBD/HbA1.html
http://www.bioinfo.de/isb/2002/02/0012/
http://ariel.ucs.unimelb.edu.au/~cotton/dsum14.htm
http://www.ncbi.nlm.nih.gov/CBBresearch/Seminar/2001spring/0726300.txt
http://www.protana.com/homePages/podtel/articles/Molbiol89.html
http://globin.cse.psu.edu/globin/html/huisman/variants/
http://www.kibb.knc.ru/biophys_lab_pub.html |
www.isrec.isb-sib.ch
HBB
HBA2
http://globin.cse.psu.edu/globin/html/docs/essay.html
Alpha thallasaemia
http://www.fhs.mcmaster.ca/grad/medsci/research/faculty/chui.htm
http://www.tau.ac.il/~racheli/teaching/bioinfo/humhbb/humhbb_omim.html
http://www.protana.com/homePages/podtel/articles/Molbiol89.html

Honors and Awards:

1963-           Silver Medal Scholarship

1969-           Award for Scholarly Outstanding

1981,1982-        Second and third Price for the scientific studies
                           of the conformational dynamic of proteins in
                           the Scientific Competition in the Engelhadt
                           Institute of Molecular Biology

1982- The Silver Medal and the Bronze Medal at
1983- the Exhibition of Achievements of the National Economy, section " Medicine for the
                               Care of Public Health”, Moscow, Russia,
1990- The First Price in the Competition for Best Poster ‘s Presentation
                          (erythrocyte membrane protein study) at the International Symposium
                        "Molecular Factors of Hematopoiesis and Stem Cells", Moscow, Russia,

LIST OF PUBLICATIONS

PREEVIEWED PUBLICATIONS IN THE PROFESSIONAL JOURNALS

2. Ames,J.B., Hamasaki,N., and Molchanova,T. (2002). Structure and calcium-binding studies of a recoverin mutant (E85Q) in an allosteric intermediate state. Biochemistry 41, 5776-5787.
3. Molchanova,T.P., Kolledey,S.V., Pronina,L.C., Mirgorodskaya,O.A., Musolyamov,A.C., Abaturov,L.V., and Huisman,T.H. (1999). Unstable Hb Newcastle [beta92(F8)His-->Pro], first case discovered in a Russian patient. Hemoglobin 23, 373-378.
4. Molchanova,T.P., Postnikov,Y., V, Gu,L.H., and Huisman,T.H. (1998). Historical note: the beta-thalassemia allele in the noble Russian family Lermontov is identified as the ATG-->ACG change in the initiation codon. Hemoglobin 22, 283-286.
5. Chui,D.H., Hardison,R., Riemer,C., Miller,W., Carver,M.F., Molchanova,T.P., Efremov,G.D., and Huisman,T.H. (1998). An electronic database of human hemoglobin variants on the World Wide Web. Blood 91, 2643-2644.
6. Hardison,R.C., Chui,D.H., Riemer,C.R., Miller,W., Carver,M.F., Molchanova,T.P., Efremov,G.D., and Huisman,T.H. (1998). Access to a syllabus of human hemoglobin variants (1996) via the World Wide Web. Hemoglobin 22, 113-127.
7. Bisse,E., Schlemer,E., Lizama,M., Huaman-Guillen,P., Wieland,H., Adam,G., Molchanova,T.P., and Huisman,T.H. (1998). Hb Strasbourg [beta 23(B5)Val-->Asp]; a high oxygen affinity variant observed in a German family. Hemoglobin 22, 69-73.
8. Smetanina,N.S., Molchanova,T.P., and Huisman,T.H. (1997). Analysis of mRNA from red cells of patients with thalassemia and hemoglobin variants. Hemoglobin 21, 437-467.
9. Molchanova,T.P. and Huisman,T.H. (1997). The levels of abnormal hemoglobin in persons with heterozygosities for an alpha chain variant and for beta-thalassemia. Hemoglobin 21, 173-177.
10. Molchanova,T.P. and Huisman,T.H. (1996). The importance of the 3' untranslated region for the expression of the alpha-globin genes. Hemoglobin 20, 41-54.
11. Molchanova,T.P., Smetanina,N.S., and Huisman,T.H. (1995). A second, elongated, alpha 2-globin mRNA is present in reticulocytes from normal persons and subjects with terminating codon or poly A mutations. Biochem. Biophys. Res. Commun. 214, 1184-1190.
12. Harthoorn-Lasthuizen,E.J., Nabben,F.A., Kazanetz,E.G., Gu,L.H., Molchanova,T.P., and Huisman,T.H. (1995). HB Mizuho or alpha 2 beta 2 68(E12)Leu-->Pro in a young Dutch boy. Hemoglobin 19, 203-206.
13. Prchal,J.T., Adler,B., Wilson,J.B., Baysal,E., Qin,W.B., Molchanova,T.P., Pobedimskaya,D.D., Kazanetz,E.G., and Huisman,T.H. (1995). Hb Bibba or alpha 2 136(H19)Leu-->Pro beta 2 in a Caucasian family from Alabama. Hemoglobin 19, 151-164.
14. Pobedimskaya,D.D., Molchanova,T.P., Streichman,S., and Huisman,T.H. (1994). Compound heterozygosity for two alpha-globin gene defects, Hb Taybe (alpha 1; 38 or 39 minus Thr) and a poly A mutation (alpha 2; AATAAA-- >AATAAG), results in a severe hemolytic anemia. Am. J. Hematol. 47, 198-202.
15. Molchanova,T.P., Pobedimskaya,D.D., and Huisman,T.H. (1994). The differences in quantities of alpha2- and alpha1-globin gene variants in heterozygotes. Br. J. Haematol. 88, 300-306.
16. Pobedimskaya,D.D., Molchanova,T.P., and Huisman,T.H. (1994). Hb Ramona or alpha (2)24(B5)Tyr-->Cys beta 2. Hemoglobin 18, 365-366.
17. Qin,W.B., Pobedimskaya,D.D., Molchanova,T.P., Wilson,J.B., Gu,L.H., de Pablos,J.M., and Huisman,T.H. (1994). Hb Fannin-Lubbock in five Spanish families is characterized by two mutations: beta 111 GTC-->CTC (Val-->Leu) and beta 119 GGC-->GAC (Gly-- >Asp). Hemoglobin 18, 297-306.
18. Curuk,M.A., Molchanova,T.P., Postnikov,Y., Pobedimskaya,D.D., Liang,R., Baysal,E., Kolodey,S., Smetanina,N.S., Tokarev,Y., Rumyantsev,A.G., and . (1994). Beta-thalassemia alleles and unstable hemoglobin types among Russian pediatric patients. Am. J. Hematol. 46, 329-332.
19. Molchanova,T.P., Pobedimskaya,D.D., and Postnikov,Y. (1994). A simplified procedure for sequencing amplified DNA containing the alp. Hemoglobin 18, 251-255.
20. Molchanova,T.P., Pobedimskaya,D.D., Ye,Z., and Huisman,T.H. (1994). Two different mutations in codon 68 are observed in Hb G-Philadelphia heterozygotes. Am. J. Hematol. 45, 345-346.
21. Qin,W.B., Baysal,E., Wong,K.F., Molchanova,T.P., Pobedimskaya,D.D., Sharma,S., Wilson,J.B., and Huisman,T.H. (1994). Quantities of alpha Q chain variants in heterozygotes with and without a concomitant beta-thalassemia trait. Am. J. Hematol. 45, 91-93.
22. Dincol,G., Dincol,K., Erdem,S., Pobedimskaya,D.D., Molchanova,T.P., Ye,Z., Webber,B.B., Wilson,J.B., and Huisman,T.H. (1994). Hb Capa or alpha (2)94(G1)Asp-->Gly beta 2, a mildly unstable variant with an A-->G (GAC-->GGC) mutation in codon 94 of the alpha 1-globin gene. Hemoglobin 18 , 57-60.
23. Curuk,M.A., Dimovski,A.J., Baysal,E., Gu,L.H., Kutlar,F., Molchanova,T.P., Webber,B.B., Altay,C., Gurgey,A., and Huisman,T.H. (1993). Hb Adana or alpha 2(59)(E8)Gly-->Asp beta 2, a severely unstable alpha 1-globin variant, observed in combination with the -(alpha)20.5 Kb alpha-thal-1 deletion in two Turkish patients. Am. J. Hematol. 44, 270-275.
24. Pobedimskaya,D.D., Molchanova,T.P., Huisman,T.H., Harding,S.R., and Bakanec,R. (1993). Hb F-Saskatoon or alpha 2G gamma (2)21(B3)Glu-->Lys observed in a North American Indian newborn. Hemoglobin 17, 547-549.
25. Pobedimskaya,D.D., Molchanova,T.P., Amernick,R., Druskin,M.S., Webber,B.B., Wilson,J.B., and Huisman,T.H. (1993). Hb Sinai-Baltimore or alpha 2 beta (2)18(A15)Val->Gly, a silent, mildly unstable beta chain variant detected by isoelectrofocusing and high performance liquid chromatography. Hemoglobin 17, 505-512.
26. Qin,W.B., Ju,T.L., Yue,X.L., Yan,X.L., Qin,L.Y., Molchanova,T.P., Pobedimskaya,D.D., and Huisman,T.H. (1993). Hb A2-liangcheng [delta 117(G19)Asn->Asp(AAC->GAC)]: a new delta chain variant detected by gene analysis in a Chinese family. Hemoglobin 17, 463-466.
27. Malcorra-Azpiazu,J.J., Wilson,J.B., Molchanova,T.P., Pobedimskaya,D.D., and Huisman,T.H. (1993). Hb Porto Alegre or alpha 2 beta 29(A6)Ser->Cys in unrelated families of the Canary Islands. Hemoglobin 17, 457-461.
28. Postnikov,Y., Molchanova,T.P., and Huisman,T.H. (1993). Allele-specific amplification for the identification of several hemoglobin variants. Hemoglobin 17, 439-452.
29. Gu,L.H., Wilson,J.B., Molchanova,T.P., McKie,K.M., McKie,V.C., and Huisman,T.H. (1993). Three sickle cell anemia patients each with a different alpha chain variant. Diagnostic complications. Hemoglobin 17 , 295-301.
30. Divoky,V., Svobodova,M., Indrak,K., Chrobak,L., Molchanova,T.P., and Huisman,T.H. (1993). Hb Hradec Kralove (Hb HK) or alpha 2 beta 2 115(G17)Ala-->Asp, a severely unstable hemoglobin variant resulting in a dominant beta- thalassemia trait in a Czech family. Hemoglobin 17, 319-328.
31. Molchanova,T.P., Postnikov,Y.V., Gu,L.H., and Huisman,T.H. (1993). Hb A2-Grovetown or alpha 2 delta (2)75(E19)Leu-->Val. Hemoglobin 17, 289-291.
32. Molchanova,T.P., Postnikov,Y., Wilson,J.B., Webber,B.B., Gu,L.H., Sabio,H., Waldron,P., and Huisman,T.H. (1993). Hb Madrid or alpha 2 beta (2)115(G17)Ala-->Pro in a black teenager. Hemoglobin 17, 251-254.
33. Molchanova,T.P., Postnikov,Y., Gu,L.H., Prior,J.F., Raven,J.L., Bennett,J.A., and Huisman,T.H. (1993). Hb Tigraye or alpha 2 beta (2)79(EF3)Asp-->His(GAC-->CAC): a hemoglobin variant with increased oxygen affinity observed in an Ethiopian male. Hemoglobin 17, 247-250.
34. Molchanova,T.P., Postnikov,Y., Pobedimskaya,D.D., Smetanina,N.S., Moschan,A.A., Kazanetz,E.G., Tokarev,Y., and Huisman,T.H. (1993). Hb Alesha or alpha 2 beta (2)67(E11)Val-->Met: a new unstable hemoglobin variant identified through sequencing of amplified DNA. Hemoglobin 17, 217-225.
35. Pobedimskaya,D.D., Molchanova,T.P., Gu,L.H., Molina,M.A., de Pablos,J.M., and Huisman,T.H. (1993). Hb F-Sacromonte or alpha 2G gamma (2)59(E3)Lys-->Gln observed in a Spanish newborn and his mother. Hemoglobin 17, 269-274.
36. Molchanova,T.P. (1993). A new screening test for unstable hemoglobins using N-butanol and red blood cells. Hemoglobin 17, 81-84.
37. Smetanina,N.S., Zhestkova,N.M., Molchanova,T.P., and Tokarev,I. (1993). [Koln hemoglobinopathy: 3 patients in one Russian family]. Ter. Arkh. 65, 45-48.
38. Molchanova,T.P., Wilson,J.B., Gu,L.H., Guemira,F., Fattoum,S., and Huisman,T.H. (1992). Hb Bab-Saadoun or alpha 2 beta (2)48(CD7)Leu----Pro, a mildly unstable variant found in an Arabian boy from Tunisia. Hemoglobin 16, 267-273.
39. Negri,A.S., Maldonado Eloy-Garcia,J., Molchanova,T.P., Wilson,J.B., Gu,L.H., and Huisman,T.H. (1992). Hb Brockton [alpha 2 beta 2(138)(H16)Ala-->Pro] observed in a Spanish girl. Hemoglobin 16, 511-514.
40. Liu,J.S., Molchanova,T.P., Gu,L.H., Wilson,J.B., Hopmeier,P., Schnedl,W., Balaun,E., Krejs,G.J., and Huisman,T.H. (1992). Hb Graz or alpha 2 beta 2(2)(NA2)His-->Leu; a new beta chain variant observed in four families from southern Austria. Hemoglobin 16, 493-501.
41. Molchanova,T.P., Wilson,J.B., Gu,L.H., Hain,R.D., Chang,L.S., Poon,A.O., and Huisman,T.H. (1992). A second observation of the fetal methemoglobin variant Hb F-M-Fort Ripley or alpha 2G gamma 2(92)(F8)His----Tyr. Hemoglobin 16, 389-398.
42. Dmitrieva,M.G., Kazanets,E.G., Molchanova,T.P., Karpova,I.V., Sevast'ianova,M.G., and Andreeva,A.P. (1991). [Oxygen-binding properties of blood in hemoglobinosis M Boston detected in the USSR for the first time]. Gematol. Transfuziol. 36, 9-11.
43. Molchanova,T.P., Kolodei,S.V., and Tokarev,Y. (1991). Expression of human membrane proteins 4.1a and 4.1b in reticulocytes and mature erythrocytes. Biomed. Sci. 2, 379-384.
44. Tasheva,E.S., Zareva,Z.Z., Topuzova,S.T., and Molchanova,T.P. (1990). Hb Andrew-Minneapolis [beta 144(HC1)Lys----Asn] in a Bulgarian family. Hemoglobin 14, 227-228.
45. Molchanova,T.P. (1989). [Basis of the molecular organization of erythrocyte membrane proteins and their defects leading to hemolytic anemia]. Gematol. Transfuziol. 34, 32-41.
46. Molchanova,T.P., Mirgorodskaia,O.A., Abaturov,L.V., Podtelezhnikov,A.V., and Tokarev,I. (1989). [Location of amino acid substitutions in human hemoglobin. Mass spectrometric rapid analysis of tryptic peptides]. Mol. Biol. (Mosk) 23, 225-239.
47. Molchanova,T.P. (1989). [Detection of unstable hemoglobins with the use of normal butyl alcohol]. Lab Delo 29-31.
48. Molchanova,T.P. and Tokarev,I. (1988). [Effect of various factors on the destruction of abnormal hemoglobins in vitro. A screening method for the detection of unstable abnormal human hemoglobins]. Gematol. Transfuziol. 33, 39-43.
49. Kazanets,E.G., Andreeva,A.P., Molchanova,T.P., Pronina,L.K., and Tokarev,I. (1988). [First cases of the detection of Hb Hyde Park in the Soviet Union]. Gematol. Transfuziol. 33, 43-46.
50. Idel'son,L.I., Molchanova,T.P., Aseeva,E.A., Spivak,V.A., and Tokarev,I. (1987). [A second case of an Hb Takoma carrier in Moscow]. Gematol. Transfuziol. 32, 45-46.
51. Molchanova,T.P., Zareva,Z.Z., Rashkov,R.G., Abaturov,L.V., and Tokarev,I. (1987). [A first case of Hb J Paris-1 alpha2[A10] Ala----Asp beta2 carrier in Bulgaria. Structural organization]. Gematol. Transfuziol. 32, 15-19.
52. Molchanova,T.P. (1987). [Functional role of proteolysis in erythroid cells in hemoglobinopathies]. Gematol. Transfuziol. 32, 33-39.
53. Molchanova,T.P. (1986). [Improved methods for the laboratory diagnosis of unstable human hemoglobin anomalies]. Gematol. Transfuziol. 31, 43-45.
54. Dubrova,I., Ikramov,K.M., Altukhov,I., Spivak,V.A., and Molchanova,T.P. (1985). [Genetic and clinical study of a family with abnormal hemoglobin Hb D Punjab 121 beta Glu----Gln]. Genetika 21, 1918-1920.
55. Molchanova,T.P., Malozemova,N.G., and Abaturov,L.V. (1982). [Proteolytic degradation of native hemoglobin and its constituent parts- -isolated subunits and globin. I. Kinetic data and the character of the process of the breakdown of native forms]. Mol. Biol. (Mosk) 16, 1128-1143.
56. Abaturov,L.A. and Molchanova,T.P. (1982). [Proteolytic degradation of native hemoglobin and its constituent parts- -isolated subunits and globin. II. Correlation with the hydrogen exchange data and the mechanism of the burst-like proteolytic breakdown of native proteins]. Mol. Biol. (Mosk) 16, 1144-1164.
57. Spivak,V.A., Molchanova,T.P., Ermakov,N.V., Postnikov,I., and Tokarev,I. (1982). [Hemoglobin Lepore Boston in Bulgaria]. Probl. Gematol. Pereliv. Krovi. 27, 27-31.
58. Spivak,V.A., Molchanova,T.P., Postnikov,Y., Aseeva,E.A., Lutsenko,I.N., and Tokarev,Y. (1982). A new abnormal hemoglobin: Hb Mozhaisk beta 92(F8)His leads to Arg. Hemoglobin 6, 169-181.
59. Spivak,V.A., Molchanova,T.P., Ermakov,N.V., Tokarev,Y., Martinez,G., Szelenyi,J., Horanyi,M., Foldi,J., Hollan,S., Kazieva,H., and Shamov,I.A. (1981). A new hemoglobin variant: Hb Dagestan alpha 60(E9) Lys leads to Glu. Hemoglobin 5, 133-138.
60. Molchanova,T.P., Abaturov,L.V., Spivak,V.A., Ermakov,N.V., and Tokarev,I. (1980). [Hemoglobin M Saskatoon alpha 2 beta 2 63 (E7) His--Tyr. Structural identification, hemichrome formation and proteolytic degradation]. Mol. Biol. (Mosk) 14, 1253-1266.
61. Spivak,V.A., Molchanova,T.P., and Tokarev,I. (1980). [Rapid and highly sensitive method of detection of abnormal hemoglobins S, E and D Punjab]. Probl. Gematol. Pereliv. Krovi. 25, 55-59.
62. Spivak,V.A., Molchanova,T.P., Ermakov,N.V., Tokarev,I., and Gar'kavtseva,R.F. (1979). [Primary structure of abnormal E-like hemoglobin]. Biokhimiia. 44, 816-821.
63. Spivak,V.A., Molchanova,T.P., Ermakov,N.V., Tokarev,I., Gar'kavtseva,R.F., and Asanov,I. (1978). [Determination of a primary structure of abnormal D-like hemoglobin isolated from a blood donor]. Biokhimiia. 43, 1175-1183.
64. Abaturov,L.V., Yakobashvily,N.N., Jinoria,K.S., Molchanova,T.P., and Varshavsky,Y.M. (1976). Effect of inter-subunit contact on intramolecular conformational motility (conformational stability) of hemoglobin as revealed by hydrogen exchange. FEBS Lett. 70, 127-130.

BOOKS:
1. Smetanina NS, Molchanova TP, Pobedimskaya DD, Kazanezh EG, Tokarev Yu.N., Rumyantsev AG: Molecular-Genetic of the Hemoglobinopathies from children.   Practical Recommendations and Protocols for Diagnosis, 2001, Russian Ministry of Health
2. Abaturov L.V., Lebedev Yu.O., Molchanova T.P. et al.: Conformational rigidity and fluctuational motility of the global proteins: water as a stabilizer and plastificator of structure.- In the Book "Equilibrium Dynamics of the Biopolymer Structure", Pushino, Russia, 1990, pp.49-77 (in Russian).
3.      Abaturov L.V., Molchanova T.P.: Dynamic structure of proteins according to the data of the hydrogen exchange and proteolytic degradation- In the Book:"Equilibrium Dynamics of the Native Structure of Protein", E.A. Burshtein, ed., Pushino, 1977, pp.5-25 ( in Russian).

ABSTRACTS

1. Katagiri NH, Molchanova TP, Ames JB: Cloning and characterization of a fission yeast homolog of recoverin processing a calcium-myristoyl switch- Kyoto Pombe Meeting, Japan 2002

2. Molchanova T.P., Smetanina N.S., and THJ Huisman : The analisis of mRNA from red cells of patients with thalassemia and hemoglobin vriants- 8-th Annual Thalassemia Parent and Patients International Conference, Malta, April, 1997.

3. Hardison R.C., Reimer C., Miller W., Chui D.H.K., Molchanova T.P., Carver M.F.H., Efremov G.D., Baysal E., and T.H.J. Huisman: Electronical and printed Syllabi describing 1 human hemoglobin variants and (2) determinants of thalassemia and HPFH – HUGO 4th International Mutation Database Meeting, Baltimore, U.S.A., October, 1997.

4. Molchanova T.P., and T.H.J. Huisman: Human hemoglobin variants: a gene spesific database-HUGO Mutation Database MeetingSan Francisco, October, 1996

5. Molchanova, T.P., Kolodey, S.V., Tokarev, Yu.N.: Human erythrocyte membrane proteins 4.1a and 4.1b in terminal stages of erythropoiesis. Abst. Symp. Molecular Factors of Hematopoiesis and Stem Cells, Moscow, 1990, p. 76.

6. Molchanova T.P., Mirgorodskaya O.A., Surin V.L. et al.: Mass-spectrometry and polymerase chain reaction in the human abnormal hemoglobin identification. - 1st All-Union Symposium on Human Genome, Pereslavl-Zalevski, Russia, 1990, pp. 174-175.

7. Abaturov L.V., Lebedev Yu.o., Molchanova T.P. et al. : The conformational mobility and flexibility of the protein structure. The role of the hydration in the protein structural mobility. - 14th International Congress of Biochemistry, Prague, Czechoslovakia, 1988.

8. Molchanova T.P., Abaturov L.V.: The influence of the point amino acid substitution in the active center of the human hemoglobin on the Hb's   conformational stability and on Hb's affinity to the external ligands.- VI Conference on Spectroscopy of the Biopolymers, Kharkov, 1988.

9. Molchanova T.P., Mirgorodskaya O.A., Eremiyantz A.R. et al.: First case of carrier of unstable hemoglobin Hb Louisville b 42 (CD1) Phe-->Leu in two carriers from Moscow family.- Belorussian Congress of Hematologists and Transfuziologists, Minsk, 1988

10. Molchanova T.P., Tokarev Yu.N., Murgorodskaya O.A. et al. : Mass-spectrometric express analysis of the tyiptic peptides of human hemoglobin.- VII All-Union Symposium on Chemistry of Proteins and Peptides, Riga, 1987.

11. Molchanova T.P., Abaturov L.V., Tokarev Yu.N.: Role of the substitution of the external residues in the conformational stability of human hemoglobin- Symposium on Phisico-chemical Properties of the Biopolimers in Solutions and Cells with the participation of the contries of the Council for Mutual Economic Aid, Pushino, Russia, 1986, p. 113-114.

12. Molchanova T.P., Abaturov L.V., Tasheva E.S., Tokarev Yu.N.: Increased thermal denaturation of the metform of Hb O Arab ? 121 (GH4) Glu-->Lys - II National Congress on Medical Biology and Genetics, Sofia, 1985, p.76.

13. Spivak V.A., Molchanova T.P., Aseeva E.A., Luztenko I.N., Postnikov Yu.V. : Molecular diagnosis and physico-chemical studies of the human abnormal hemoglobins. - 2nd All-Union Symposium of Hematologists and Transfuziologists, L'vov, 1985, pp. 357-358.

14. Molchanova T.P., Spivak V.A., Tokarev Yu.N. : The study of the proteolytic degradation of the human abnormal hemoglobins. - 6th All-Union Symposium on Chemistry of Proteins and Peptides, Riga, 1983, pp.139-140

15. Spivak V.A., Molchanova T.P., Aseeva E.A., Postnikov Yu.V. et al.: Studies of the influence of the amino acid substitution on the structure and clinical manifestation of the human abnormal hemoglobins. - 6th All-Union Symposium on Chemistry of Proteins and Peptides, Riga, 1983, pp.137-138.

16. Spivak V.A., Molchanova T.P., Aseeva E.A., Postnikov Yu.V. et al. : Electroporesis and chromatography in studies of human abnormal hemoglobins. - 6th All-Union Symposium on Chemistry of Proteins and Peptides, Riga, 1983, p.138.

17. Molchanova T.P., Spivak V.A. : The application of the ion exchange chromatography for the abnormal hemoglobin studies. - 6th All-Union Symposium on Chemistry of Proteins and Peptides, Riga, 1983, p.129

18. Spivak V.A., Molchanova T.P., Postnikov Yu.V. et al.: Molecular diagnosis of the hemoglobin Buenos Aires ? 85 (F1) Phe-->Ser and studies of its properties. - 55th Scientific conference of the All-Union Hematological Scientific Center, Moscow, 1983, pp. 76-78.

19. Molchanova T.P., Abaturov L.V. : The mechanism of the burst-like proteolytic degradation of the native proteins. - All-Union Biophysical Congress, Moscow, 1982, p. 156.

20. Nosova N.G., Molchanova T.P., Abaturov L.V. : The influence of the media on the conformational and dynamic structure of the hemoglobin. - All-Union Biophysical Congress, Moscow, 1982, pp.117-118.

21. Spivak V.A., Molchanova T.P., Postnikov Yu.V. et al. : Molecular diagnosis of abnormal hemoglobins. - 53d Scientific conference of the All-Union Hematological Scientific Center, Moscow, 1981, p. 115.

22. Tokarev Yu.N., Kolodey S.V., Postnikov Yu.V., Molchanova T.P. et al.: Distribution and heterogenety of the hereditary hemoglobinopathies in some regions of the Central Asia and Caucases. - 53d Scientific conference of the All-Union Hematological Scientific Center, Moscow, 1981, p. 101.

23. Abaturov L.V., Molchanova T.P., Dzinoria K.Sh., Lebedev Yu.O., Yakobashvili N.N.: Dynamic model of the global protein structure - All-Union Symposium on Macromoleculars of Cell. Structure, Function, Interactions, Moscow, 1979, p. 44.

24. Spivak V.A., Molchanova T.P., Ermakov N.V., Tokarev Yu.N. : Study of the primary structure of abnormal hemoglobins. - 1st All-Union Symposium of Hematologists and Transfuziologists, Baku, 1979, p. 205.

25. 5. Abaturov L.V., Molchanova T.P., Dzinoria K.Sh., Lebedev Yu.O., Yakobashvili N.N.: Dynamic model of the global protein structure - All-Union Symposium on Macromoleculars of Cell. Structure, Function, Interactions, Moscow, 1979, p. 44.

26. 6. Spivak V.A., Molchanova T.P., Ermakov N.V., Tokarev Yu.N. : Study of the primary structure of abnormal hemoglobins. - 1st All-Union Symposium of Hematologists and Transfuziologists, Baku, 1979, p. 205.

27. Molchanova T.P., Spivak V.A., Abaturov L.V. et al.: Hemoglobin M Saskatoon, molecular diagnosis, hemichrome formation and proteolytic degradation. - 1st All-Union Symposium of Hematologists and Transfuziologists, Baku, 1979, p. 218.

28. Abaturov L.V., Molchanova T.P.: The connection between genetics and conformational stability of human abhormal hemoglobins. - All-Union Conference on the Mechanisms of Regulation in the Blood System, Krasnoyarsk, Russia, 1978, pp. 127-128.

29. Abaturov L.V., Molchanova T.P.: The fewer number of detected alpha chain point mutations in comparison with beta chain mutants of human hemoglobins as a result of the difference in conformational stability of normal chains- XIV International Congress of Genetics, Moscow, 1978, part 1, p. 25.
30. Spivak V.A., Molchanova T.P., Ermakov N.V. et al. : Determination of the primary structure of the D-like abnormal hemoglobin found in donor blood. - 1st Republican Conference on Hemolytic Anemias, Dushanbe, 1976, pp. 111-112.

31. Abaturov L.V., Molchanova T.P., Dzinoria K.Sh. et al. : Intramolecular mobility different ligand forms of human hemoglobin, its constituent pats: globin and sibunits according to the data of the hydrogen exchange and proteolytic degradation. - XI Mendeleev Symposium on General and Applied Chemistry, Almaata, 1975, p.40.

32. Molchanova T.P., Malozemova H.G., Abaturov L.V. : Ptoteolytic degradation of the hemoglobin and its constituent parts. - Conference on Conformational Changes of Biopolymers in Solutions, Tbilisi, 1975, pp.113-114.

33. Molchanova T.P., Tatishvili D.A., Abaturov L.V., Varshavski Ya.M. : Some structural features of the hemichromes as an intermediate form in the course of denaturation and spontneouse inactivation of hemoglobin. - Soviet-French Symposium on the Physical Chemistry of Proteins and Peptides, Pushino, Russia, 1975, p.38.

34. Abaturov L.V., Yakobashvily N.N., Jinoriya K.Sh., Molchanova T.P., Varshavsky Ya.M.: Effect of inter-subunit contact on intramolecular conformational motility (conformational stability) of hemoglobin as related by hydrogen exchange- Abst.Commun. 9th FEBS Meeting, Budapest, 1974, p.350

REVIEWS:
1. Molchanova T.P., T.H.J. Huisman - Hemoglobinopathies in Russia. Review (former USSR), Balkan J. Med. Gen., 1 (4), 143-148, 1998.
2. Molchanova, T.P., Postnikov, Yu.V.: Hemoglobinopathies in the former Soviet Union (Review). Sphere, 15(3): 1-4, 1992.
3. Molchanova, T.P., Tokarev, Yu.N.: Abnormal Hemoglobins in the USSR (Review). In: "Hematology Reviews" (ed. by O.K. Gavrilov), Harvard Acad. Press, v. 2B, pp. 1-32, 1990.
4. Molchanova, T.P.: The basis of molecular organisation of the erythrocyte membrane proteins and their defects in hemolytic anemias (Review). - Gematol. and Transfus., 34: 32-41, 1989 (Russian ).
5. Molchanova, T.P.: The functional role of proteolysis in erythroid cells (Review). Gematol. and Transfus., 32(3): 33-39, 1987 (Russian).

Tatiana Molchanova